ABSTRACT

Each year, the American Mosquito Control Association (AMCA) annual meeting features a program designed to highlight the various research, collaborations, and innovations that impact our programs throughout the USA. The AMCA meeting in San Juan, Puerto Rico, from March 3 to 7, 2025, gave our members and local professionals a new perspective on the battle against mosquito-borne diseases. In recent years, there have been local outbreaks of dengue fever, Zika virus, and chikungunya virus in this US territory. The mosquito control professionals and local citizens welcome, support, and collaborate and may also benefit from the national and international meeting. We thank the program committee, local arrangement committee, and AMCA Board for their collaboration in making the meeting attractive, which resulted in more than 970 registrations worldwide. The European Mosquito Control Association showcased European expertise in mosquito control at this event, strengthening ties with AMCA. With the rapid development of technology, including artificial intelligence, robotic techniques, and tools, mosquito control professionals will face new challenges and opportunities for exploration. I want to take this opportunity to promote and encourage members to adopt best management practices for the effective use of technology in the surveillance and control of mosquitoes and other disease vectors.

ABSTRACT

The mosquito species commonly named Henderson American pointy mosquito, Aedes hendersoni, was found during a routine mosquito vector surveillance from 2020 to 2023 in diverse collection sites with conserved forested vegetation at the Eco-Parks Rincón de la Silla and El Salto in Nuevo León, northeastern Mexico. Collected specimens were labeled, mounted, and identified using taxonomic keys. Further confirmation of Ae. hendersoni was performed by deoxyribonucleic acid (DNA) barcoding and cytochrome oxidase-I (COI) sequences served to confirm the identity of Ae. hendersoni by comparison with other sequences of species with Triseriatus group and Protomacleaya subgenus of Aedes. Voucher specimens were curated and are available in 2 Mexican collection repositories and COI sequences were deposited in the NCBI GenBank with accession number MG242473.1. Hence, the presence of Ae. hendersoni in México is documented for the first time. Now, geographic occurrence of this species is from Canada throughout United States to northeastern México. With this new record, there are the 4 mosquito species in the Triseriatus group [Aedes: (Protomacleaya)] and 252 species in México.

ABSTRACT

Plant-based insecticides are getting attention as an alternative mosquito control strategy because of the emergence of insect resistance to currently used synthetic chemicals. Further, their high safety profile makes them ideal candidates for environmental applications. The current study evaluated the insecticidal potential of Psidium guajava and Piper betle leaf extracts against Aedes aegypti through in vitro and in silico approaches. In laboratory studies, the LC₅₀ of n-hexane extract of P. guajava and ethyl acetate extract of P. betle were 95.21 ppm and 217.7 ppm after 24-h exposure, respectively. The gas chromatography-mass spectrometry analysis identified important bioactive compounds, including caryophyllene (21.2%), globulol (19.9%), squalene (8.3%), and γ-muurolene (6.6%) in P. guajava and hydroxychavicol (57%), 5-allyl-2-hydroxyphenyl acetate (5.6%), phytol (2.3%), and safrole (1.8%) in P. betle extract. In silico analysis of these compounds with target proteins acetylcholinesterase (AChE), S-adenosylhomocysteine hydrolase (SAHH), and sterol carrier protein-2 (SCP-2) in Ae. aegypti larvae showed that squalene from P. guajava had a higher binding affinity with AChE (–8.4 kcal/mol) compared to globulol (–7.3 kcal/mol). However, conventional hydrogen bonding, which is stronger and more stable, was observed in the globulol-AChE complex. The in silico analysis of P. betle phytochemicals demonstrated that hydroxychavicol, phytol, and safrole had binding affinities of –6.1 kcal/mol, –6.0 kcal/mol, and –6.0 kcal/mol with SAHH, respectively. A minor increase in binding affinity was observed in the safrole-SAHH complex (–6.1 kcal/mol), whereas no change was observed in the 5-allyl-2-hydroxyphenyl acetate-AChE complex (–5.9 kcal/mol) in 2-ligand binding mode. Since these bioactive compounds target the important proteins in the developmental processes of mosquito larvae, they can further be evaluated to design natural and organic insecticides against Ae. aegypti.

Professor emeritus Bruce Eldridge in 2007 (photo by Kathy Keatley Garvey).
Citation: Journal of the American Mosquito Control Association 41, 3; 10.2987/25-7235

Bruce Frederick Eldridge, a pioneering entomologist, dedicated educator, military entomologist, and beloved family man, passed away peacefully on February 5, 2025, at the age of 91 in Davis, CA. Bruce was known for his groundbreaking work in medical entomology, his commitment to public health, and his devotion to family and community. His life spanned multiple careers, advancing science, education, and service to his colleagues.

Bruce was born on March 26, 1933, in San Jose, CA, the youngest of three boys. From an early age, he demonstrated an extraordinary affinity

EVALUATING VECTECH IDX™: AI-DRIVEN IDENTIFICATION FOR ENHANCED VECTOR MANAGEMENT

Casey Hubble,

Mary Sorensen,

Keiko Parker,

Marissa Utterback,

Tyler Stuhaan,

Melissa Cooke, and

Jake Hartle

2025 AMCA PRESIDENTIAL ADDRESS: BEST MANAGEMENT PRACTICES FOR TECHNOLOGY ADOPTION FOR SURVEILLANCE AND CONTROL OF MOSQUITOES¹

ABSTRACT

THE PRESENCE OF AEDES HENDERSONI IN MEXICO

ABSTRACT

EVALUATION OF THE INSECTICIDAL POTENTIAL OF THE LEAF EXTRACTS OF PSIDIUM GUAJAVA AND PIPER BETLE AGAINST AEDES AEGYPTI LARVAE

ABSTRACT

PRE-SEASON DEPLOYMENT OF IN2CARE® MOSQUITO STATIONS TO CONTROL AEDES AEGYPTI POPULATION IN THE WEST VALLEY REGION OF SAN BERNARDINO COUNTY, CALIFORNIA

ABSTRACT

DO IT YOURSELF: EVALUATING COMMERCIAL CO₂ REGULATORS FOR SURVEILLANCE NETWORKS USING PRESSURIZED CYLINDERS

ABSTRACT

IMPROVED SURVEILLANCE OF AEDES TRISERIATUS USING THE BG-PRO TRAP: IMPLICATIONS FOR SAMPLING HOST-SEEKING LA CROSSE VIRUS VECTORS

ABSTRACT

COMPARATIVE BEHAVIORAL RESPONSES OF Β-CARYOPHYLLENE AGAINST ANOPHELES MOSQUITO SPECIES, POTENTIAL VECTORS OF MALARIA IN THAILAND

ABSTRACT

DETERMINING DISCRIMINATING CONCENTRATIONS OF TRANSFLUTHRIN AND PERMETHRIN USING THE WHO BOTTLE ASSAY AGAINST AEDES ALBOPICTUS IN THAILAND

ABSTRACT

EVALUATION OF A BIFENTHRIN-BASED RESIDUAL BARRIER TREATMENT FOR CONTROLLING AEDES TAENIORHYNCHUS POPULATIONS ON AN ISOLATED BARRIER ISLAND IN COLLIER COUNTY, FLORIDA

ABSTRACT

CDC BOTTLE BIOASSAYS FOR DETECTING INSECTICIDE RESISTANCE IN AEDES ALBOPICTUS, AEDES KOREICUS, AND CULEX PIPIENS FROM U.S. ARMY GARRISONS IN THE REPUBLIC OF KOREA (2023 AND 2024)

ABSTRACT

BRUCE FREDERICK ELDRIDGE MARCH 26, 1933 to FEBRUARY 5, 2025

EVALUATING VECTECH IDX™: AI-DRIVEN IDENTIFICATION FOR ENHANCED VECTOR MANAGEMENT

THE DEVELOPMENT OF A WATER-JACKETED MEMBRANE FEEDER REPELLENT TESTING SYSTEM FOR BLACK FLIES (DIPTERA: SIMULIIDAE)

AGDISP REFINEMENT STUDY: REPORT ON THE AERIAL PESTICIDE APPLICATION SURVEY AND SUBSEQUENT TREATMENT CHOICES

IMPACT OF THREE ESSENTIAL OILS AS ACTIVE INGREDIENTS OF TOXIC SUGAR BAITS ON FEEDING RATE AND MORTALITY OF AEDES AEGYPTI

WAYNE JOHN CRANS 1937–2025

First Records of Aedeomyia squamipennis in Palm Beach County, Florida, USA

An Inexpensive System to Investigate the Daily Rhythms of Insects

American Mosquito Control Association Board of Directors Meeting via online platform—March 20, 2020

Meeting Increased Demand for Mosquito Adulticides Containing the Active Ingredient Naled Following Hurricanes and Tropical Storms

2018 AMCA Memorial Lecture Honoree: Fred William Knapp ¹